To help you picture regions oftentimes mixed up in hereditary control over leaf rust opposition, the new QTL-analysis density directory (Chardon ainsi que al. 200cuatro) are computed (Fig. step three). So it fact develops for the a local having (i) the number of QTL out-of some other experiments, (ii) this new distance between QTL positions, and you may (iii) the precision of the individual QTL position estimation.
Fig. 3. New assessment fact is actually computed the step step one centimorgan (cM) and plotted collectively for every single chromosome (blue contour). A few lateral dotted contours could be the mediocre property value the fact (0.055) plus the threshold to possess large opinions (0.275), recognized as five times the typical worth (Chardon et al. 2004). Taverns below the overview bend portray brand new wheat chromosomes in which QTL was indeed estimated. Light gray (green) packages depict meta-QTL harboring at the very least 2 QTL, whereas dark gray (red) boxes portray unique QTL. Ranking away from prior to now mapped Lr genetics into the wheat is actually portrayed from the triangles below chromosome bars; Lr genetics colocalized which have meta-QTL try conveyed when you look at the deep gray (blue).
New assessment directory is actually calculated all the centimorgan and estimates was indeed received for each of one’s 144 projected QTL (Fig. 3). As a whole, 52 assessment peaks was in fact gotten, at which 47 surpassed the common property value this new figure (0.055) along the genome and represented genomic beautiful places to have leaf rust resistance. Of one’s 47 peaks, twenty five exhibited viewpoints exceeding the fresh large-well worth tolerance (0.275) (Fig. 3). The number of peaks exceeding the typical threshold varied from 1 in the chromosome 4A and you may 7A so you can four into the chromosomes 1B, 2D, and you can 7B. Toward second, the five peaks together with surpassed the newest large-worthy of endurance.
QTL meta-studies.
New 144 QTL projected onto the Wheat Element 2004 map have been subjected to meta-study making use of the functions away from Goffinet and Gerber (2000) if the quantity of QTL when you look at the a good chromosome try lower than 10 and the ones from Veyrieras et al. (2007) when the number is ten or more. Following a keen AIC, 141 QTL have been grouped on forty-eight MQTL (Dining table 2). The software program failed to believe about three QTL for meta-study due to their low LOD rating beliefs or highest Quand. In all, thirty five MQTL, spanning 128 QTL, was in fact caused by the brand new integration with a minimum of 2 QTL, and thirteen QTL stayed since the unique QTL (Desk dos).
a your top the main table shows MQTL spanning from the least several clustered QTL additionally the down part suggests QTL kept because book QTL.
c AUDPC = town within the state progress curve, DS = problem seriousness, EA = early aborted colonies, EST = built colonies, Web browser = issues efficiency, They = disease style of, LIA = leaf contaminated urban area, LP = hidden period, LR = leaf resistance, LS = lesion size, LTN = leaf tip necrosis, NEC = necrotic territories, SPL = spore creation each lesion, and you may SPS = spore manufacturing for every unit off sporulating muscle.
Chromosome | MQTL | Updates (cM) | Au moment ou (nine5%) | Flanking markers | QTL (n) b | Feature c | Opposition provider |
---|---|---|---|---|---|---|---|
1A | MQTL1 | Xfba254–Xwmc95b | 4 | AUDPC, DS | Apache, Sujata | ||
1B | MQTL2 | step 1.8six | Xcdo98.2–Xmwg837b | 6 | AUDPC, DS, They, LTN | Forno, MG5323, Oberkulmer, Pastor, Sujata | |
MQTL3 | 8.46 | Xbarc81–Xfba294 | 2 | AUDPC, DS | Bainong 64 | ||
MQTL4 | 2.10 | Xfba266c–XksuI27 | 2 | DS | Pastor, Pavon 76 | ||
MQTL5 | 0.28 | XksuI27–Xbarc80 | 2 | AUDPC, DS | Francolin#step one | ||
2A | MQTL6 | 4.62 | Xglk554–Xcmwg682b | 2 | AUDPC | Colosseo, Forno | |
2B | MQTL7 | 3.68 | Xgwm55–Xgwm429 | 4 | AUDPC, DS, They | Carberry, Kariega, Avocet S, W-7984 | |
MQTL8 | Xwmc265–Xgwm877.1 | 7 | AUDPC, DS, LP | Apache, Creso | |||
2D | MQTL9 | 9.thirty-two | Xgwm455–Xbcd718 | 3 | AUDPC, DS | Harmony, Forno, MN99394-1 | |
MQTL10 | 8.forty | Xfba74a–Xgwm30.step 1 | 3 | AUDPC, DS, LTN | Forno, Pastor | ||
MQTLeleven | cuatro.eleven | Xcdo1008–Xksud23a | 6 | AUDPC, DS, SPS | Balance | ||
3A | MQTL12 | six.73 | Xcdo1345–Xgwm2 | 2 | LR, LTN | Forno, Oberkulmer | |
MQTL13 | Xcfa2134–XgbxG242 | 2 | They, LIA | TA4152-60 | |||
MQTL14 | dos.thirty-two | Xmwg570–Xcdo113 | 2 | AUDPC, LIA | Lloyd | ||
3B | MQTL15 | 2.08 | XksuG30a–Xgwm533a | 9 | DS, Internet explorer, They, LIA, LP, SPL | Balance, Capo, Opata 85 | |
MQTL16 | 0.89 | Xgwm566–Xgwm888.step 3 | 3 | DS, It, LIA | Francolin#step 1, TA4152-sixty | ||
4A | MQTL17 | XgbxR247–Xpsr593.2 | 2 | DS, They | Opata 85 | ||
4B | MQTL18 | Xgwm495a–Xcsu25 | 3 | AUDPC, DS | Avocet S, Creso, Forno | ||
MQTL19 | Xgwm131.2–Xmwg11a | 2 | DS, LTN | Carberry, Oberkulmer | |||
4D | MQTL20 | 2.50 | Xbarc98–Xfbb336 | 3 | AUDPC, It, LIA | Sujata, TA4252-sixty | |
MQTL21 | Xcdo1312a–Xfba177b | 4 | AUDPC, DS, LS, SPL | Balance | |||
5B | MQTL22 | cuatro.79 | Xbarc4–Xgwm371 | 2 | AUDPC, DS | Kariega, SHA3/CBRD | |
MQTL23 | 8.83 | Xpsp3065–Xgwm118 | 5 | They, LIA, LTN | Americano 25e, Capo, Carberry, TA4152-60 | ||
6A | MQTL24 | Xmwg67.1–XksuH4.2 | 6 | AUDPC, DS, LP | Balance | ||
6B | MQTL25 | S25M19_7–Xcdo476.dos | 2 | AUDPC, DS | Bainong 64 | ||
MQTL26 | Gpc-B1–Xwmc388a | 2 | AUDPC, DS | Bainong 65 | |||
MQTL27 | Xgwm133b–Xfbb130 | 2 | DS, It | Pavon 76, TA4152-60 | |||
MQTL28 | six.91 | Xbarc134–Xwmc417b | 2 | AUDPC, DS | Kariega, Pastor | ||
7A | MQTL29 | 4.73 | XWC2028g–P78/M49.cuatro | 5 | AUDPC, DS | Balance | |
7B | MQTL30 | step three.58 | Xwmc335–Xgwm68.2 | 5 | AUDPC, DS, LIA | Colosseo, Forno, Kariega, SHA3/CBRD | |
MQTL31 | dos.17 | S25M15_19–Xgwm112 | 4 | DS, It, LTN | MG5323, Oberkulmer | ||
MQTL32 | 4.42 | Xwmc273c–Xwmc311 | 4 | AUDPC, DS, LTN | Forno, Oberkulmer, Opata 85, Pastor | ||
MQTL33 | 0.53 | Xgwm577–Xbarc340.dos | 11 | AUDPC, DS, EA, EST, LIA, LP, NEC | Colosseo, Creso, Sujata | ||
7D | MQTL34 | step 1.75 | Xbcd1438–Xcnl6b | 3 | AUDPC, DS, LTN | Forno, MN98550-5 | |
MQTL35 | nine.39 | Xgwm44–Xcfa2174c | 2 | DS, LTN | Oberkulmer, Opata 85 | ||
1A | … | 123.5 | Xwmc237–XksuE11.1a | 1 | DS | SHA3/CBRD | |
1B | … | six.thirteen | XksuF43.1–Xabc155c | 1 | LIA | Capo | |
2A | … | Xfbb329–Xgwm339 | 1 | LP | Creso | ||
… | 3.09 | Xfbb353.2–Xglk554 | 1 | LIA | Colosseo | ||
2B | … | cuatro.98 | Xwmc344–XksuD22 | 1 | LIA | Capo | |
… | 4.twenty-seven | Xfba310.1–Xbcd1231 | 1 | LIA | Capo | ||
2D | … | Xwmc243–Xgwm608.3 | 1 | DS | SHA3/CBRD | ||
5A | … | dos.00 | 5.55 | Xcnl6b–Xgwm154a | 1 | LTN | Oberkulmer |
… | Xbcd508–Xfbb255x | 1 | DS | Pastor | |||
… | Xwg114a–P43/M62-5 | 1 | EA | Creso | |||
6A | … | nine.88 | Sr26–P38/M51-4 | 1 | LTN | Oberkulmer | |
7D | … | 3.forty eight | XgbxG564–Yr18 | 1 | DS | Opata 85 | |
… | Xgwm121–Xbarc53 | 1 | LTN | Carberry |